Journal: Microorganisms
Article Title: Comparative Analysis of Salmon Cell Lines and Zebrafish Primary Cell Cultures Infection with the Fish Pathogen Piscirickettsia salmonis
doi: 10.3390/microorganisms9122516
Figure Lengend Snippet: Immunofluorescence microscopy of P. salmonis -infected salmon cell lines and zebrafish primary cell cultures. ( A ) P. salmonis -infected and mock-infected (control) salmon cell lines SHK-1 and ASK were fixated before fluorescence staining after 6 and 12 dpi. ( B ) P. salmonis -infected and mock-infected (control) zebrafish primary cultures (ZKPCC) were fixated after 1 and 5 dpi. Both panels show representative images of zebrafish cells with different cell types. For the three cultures, cellular actin was stained with phalloidin in green (Alexa Fluor 488 ® ), and the nucleus in blue with DAPI. Polyclonal antibodies against P. salmonis and a secondary antibody coupled with Alexa Fluor 594 ® marked the bacteria in red. Bar represents 50 µm. Images obtained at each channel are provided in .
Article Snippet: SHK-1, ASK and ZKPCC were seeded in 8-well chambers for microscopy visualization (Lab-TekII Chamber slide with cover RS Glass slide sterile, Thermo Fisher Scientific), using 4 × 10 4 cells/well for the cell lines, and 2 × 10 5 cells/well for ZKPCC.
Techniques: Immunofluorescence, Microscopy, Infection, Control, Fluorescence, Staining, Bacteria